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Unlocking the Power of Testosterone: Boost Muscle Gain Naturally

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Medically approved by

Maria Jacob

Last updated

Friday, January 20, 2023

A key factor in the growth of muscle mass and strength is the male sex hormone Testosterone (T). By promoting protein synthesis in muscle cells, which leads to the development of new muscle fibres, this process is achieved (1). Testosterone also increases the quantity of nuclei in muscle cells, which can promote muscle growth even more (2)

Age-related Testosterone level declines can result in a loss of muscle mass and strength. Osteoporosis, obesity, and type 2 diabetes are just a few of the health issues that can be exacerbated by low Testosterone levels (3,4).

Testosterone can be increased through a number of ways, including increasing exercise, sleep, improving one's diet, reducing stress, limiting alcohol consumption, and maintaining a healthy weight (3-5). It can also be improved using Testosterone supplements and Testosterone replacement therapy (TRT). We recommend a combination of the above for men hoping to increase T-levels and ultimately improve muscle gain. 

The role of Testosterone in building muscle

The development of strength and muscle mass depends heavily on Testosterone. It accomplishes this by promoting the process by which muscle fibres are created and repaired—muscle protein synthesis (1,2,6). Tiny tears form in the muscle fibres during activity. Then, protein synthesis is triggered to repair and rebuild these fibres, making them more robust and substantial. Faster and more effective muscle growth is facilitated by Testosterone by speeding up the rate of protein synthesis. In addition to promoting the production of muscle proteins, Testosterone contributes to the expansion of muscle cell nuclei. 

The more nuclei a muscle cell possesses, the greater potential for growth it has because nuclei are the "control centres" of cells. By encouraging the union of myoblasts, Testosterone can increase the number of nuclei in muscle cells, further accelerating muscular growth (1, 2, 6). By encouraging myogenesis, the process of producing new muscle cells, and by modifying the activity of myogenic regulatory factors (MRFs), which regulate muscle cell differentiation and proliferation, Testosterone also has an effect on muscle mass. By encouraging the development and differentiation of muscle cells, these effects lead to an increase in muscular mass and strength.

Is high Testosterone good for muscle growth?

A moderate Testosterone level is necessary for muscle building. However, having too much Testosterone may not always be advantageous (7). Acne, enlarged breasts, and an increased risk of prostate cancer are just a few of the unwanted effects of high Testosterone levels (8). Studies have also suggested that Testosterone levels that are above what is regarded as normal, or supraphysiological levels, may not always result in larger gains in strength or muscle growth. Healthy men were given supraphysiological doses of Testosterone in a study by Bhasin et al. that was published in the New England Journal of Medicine in 1996, and the results showed that while muscle size and strength increased, there was no additional benefit above normal physiological levels of Testosterone (7).

How does Testosterone contribute to muscle gain?

Given its role in protein synthesis, Testosterone might affect muscle mass by reducing muscular wasting, which is a crucial component of muscle growth (7, 8). Due to its impact on muscle wasting, Testosterone can aid in reducing muscle loss brought on by aging, chronic illnesses, and inactivity.  It's important to note that a moderate quantity of Testosterone is necessary for the growth and development of muscles. For this reason, exercises like weightlifting and resistance training, which cause muscle damage and encourage muscle protein synthesis, are very effective at boosting muscle mass and strength while also increasing the Testosterone needed to build said muscle (5).

How much Testosterone do you need to build muscle?

The ideal Testosterone concentration for muscular growth is not well-defined and can differ from person to person. A modest Testosterone level is typically thought to be necessary for muscular growth. In healthy adult men, the normal range of Testosterone is typically thought to be between 300 and 1000 nanograms per decilitre (ng/dL) (9). It's not always possible to claim that a specific level of Testosterone is necessary for muscle growth, as it can fluctuate depending on your age and other circumstances (5).

Can you build muscle with low Testosterone levels?

Given its importance to muscle building, it is much more difficult to build muscle with low T-levels (5). However, there are several strategies that one can employ to make this process easier:

  • Exercise: Even with low Testosterone levels, resistance training, such as weightlifting and high-intensity interval training (HIIT), can help to develop muscle (3-5). To encourage muscular growth and adaptation, concentrate on progressive loading by increasing the weight, reps, or sets of workouts. 
  • Nutrition: A diet rich in protein, in particular, can encourage muscle growth. To encourage muscle regeneration and recovery, consume plenty of lean protein sources, such as chicken, fish, and lentils. 
  • The importance of enough sleep for overall health and muscular development cannot be overstated (3-5). In order to encourage muscle healing and growth, one should aim for 7-9 hours of good sleep each night.
  • Speak with a healthcare expert: A healthcare practitioner can help you discover and treat the underlying medical disorders that may be causing your low Testosterone levels (3-5). To help boost Testosterone levels and support muscular growth, TRT may occasionally be recommended and your practitioner may also want to address the underlying causes of T-deficiency. 



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What happens to my muscles and body with low T?

Low Testosterone levels can result in a loss of muscle mass and strength as well as making it harder to make more muscle  (3-5). This may result in a loss of muscle mass, giving the appearance of a weaker, less toned body as well as a decrease in overall strength or power of muscles. Other changes to the body can also occur:

  • Testosterone plays a role in the management of fat mass (10). Low Testosterone levels can increase body fat, especially in the abdominal region, which raises the risk of obesity and metabolic illnesses. 
  • Bone density: Since low levels of Testosterone can result in lower bone density, which raises the risk of osteoporosis and bone fractures, Testosterone plays a function in bone metabolism (11)
  • Testosterone is important for sexual function (3,4). Low levels of Testosterone can cause erectile dysfunction, decreased sperm count, and diminished libido.

Could TestosteroneLow Testosterone levels replacement therapy help?

The hormone replacement therapy (HRT) known as Testosterone replacement therapy (TRT) involves replacing the body's naturally occurring hormones with artificial hormones made outside the body. TRT has been significantly more accessible in recent years because it is now offered in oral tablet, cream, and injection formats (12). TRT is specifically designed for men who have a natural hypogonadism or lack Testosterone.

It's also crucial to remember that Testosterone by itself won't help people gain muscle; they need to exercise and eat right in order to do so. Additionally, taking Testosterone supplements including TRT may have a number of negative side effects and risks, described in detail below. 

Risks of increasing Testosterone levels

Increasing Testosterone levels might have hazards, especially when done without competent medical supervision through hormone therapy or supplements. The following are a few potential risks (3-5,6-8,13)

  • Prostate cancer: Having high Testosterone levels can make you more likely to get prostate cancer.
  • Acne: High Testosterone levels can result in acne and other skin conditions.
  • Heart disease: By boosting blood pressure and cholesterol levels, high amounts of Testosterone can raise the risk of developing heart disease. 
  • Liver toxicity: Prolonged usage of Testosterone dietary supplements may harm the liver. 
  • Sleep Apnea: High Testosterone levels can result in sleep apnea, a condition in which a person's breathing is continuously interrupted while they are asleep.
  • Fertility: High amounts of Testosterone can cause sperm motility and count to decline, which can result in reproductive problems. 
  • Blood clots: Excessive Testosterone can result in an increase in red blood cells, which can thicken the blood and raise the risk of blood clots and cancer. This condition is known as polycythemia. 
  • Gynecomastia: Also known as gynecomastia, high Testosterone levels can cause the development of breast tissue in men.

Potential side effects of TRT

According to Mayo Clinic, the following possible side effects are frequent: 

  • Blemishes on the skin 
  • Blood in the urine  
  • Difficulty peeing
  • Frequent urination
  • Burning, itching, numbness, or tingling sensations at the application site 
  • Pimples or acne
  • Exhaustion

While there are a number of adverse effects associated with using Testosterone cream, many of the consequences of low Testosterone can exceed the dangers associated with TRT.



In conclusion, by promoting muscle protein synthesis, raising the number of nuclei in muscle cells, and controlling muscle wasting, Testosterone is essential for muscular growth and development. While utilising Testosterone supplements without a prescription is not advised, a modest dose of Testosterone is necessary for muscular growth. Having a moderate amount of Testosterone is necessary for muscle building, but having too much Testosterone might have undesirable side effects as well. Prior to beginning any hormone therapy or supplementation, you should seek medical advice from a qualified practitioner. Although it can be more difficult when low Testosterone levels are present, muscular growth is still achievable. You could also take a well-rounded approach to muscle building that includes exercise, a healthy diet, and professional advice.


  1.  Griggs RC, Kingston WI, Jozefowicz RF, Herr BE, Forbes GI, Halliday DA. Effect of Testosterone on muscle mass and muscle protein synthesis. Journal of Applied Physiology. 1989 Jan 1;66(1):498-503.
  2. Sheffield-Moore M. Androgens and the control of skeletal muscle protein synthesis. Annals of medicine. 2000 Jan 1;32(3):181-6.
  3. Isidori, A. and A. Lenzi, Risk factors for androgen decline in older males: lifestyle, chronic diseases and drugs. Journal of endocrinological investigation, 2005. 28(3 Suppl): p. 14-22.
  4. Ko, D.H., S.E. Kim, and J.Y. Lee. Prevalence of Low Testosterone According to Health Behavior in Older Adults Men. in Healthcare. 2020. MDPI.
  5. Riachy, R., K. McKinney, and D.R. Tuvdendorj, Various Factors May Modulate the Effect of Exercise on Testosterone Levels in Men. J Funct Morphol Kinesiol, 2020. 5(4).
  6.  Sheffield-Moore M. Androgens and the control of skeletal muscle protein synthesis. Annals of medicine. 2000 Jan 1;32(3):181-6.
  7. Bhasin S, Storer TW, Berman N, Callegari C, Clevenger B, Phillips J, Bunnell TJ, Tricker R, Shirazi A, Casaburi R. The effects of supraphysiologic doses of Testosterone on muscle size and strength in normal men. New England Journal of Medicine. 1996 Jul 4;335(1):1-7.
  8.  WebMD. Testosterone Cypionate Vial - Uses, Side Effects, and More. Available from: https://www.webmd.com/drugs/2/drug-5185/Testosterone-cypionate-intramuscular/details.
  9.  Zhu A, Andino J, Daignault-Newton S, Chopra Z, Sarma A, Dupree JM. What is a normal Testosterone level for young men? Rethinking the 300 ng/dL cutoff for Testosterone deficiency in men 20-44 years old. The Journal of Urology. 2022 Dec;208(6):1295-302.
  10.    Whittaker, J. and K. Wu, Low-fat diets and Testosterone in men: Systematic review and meta-analysis of intervention studies. The Journal of Steroid Biochemistry and Molecular Biology, 2021. 210: p. 105878.
  11. Snyder PJ, Kopperdahl DL, Stephens-Shields AJ, Ellenberg SS, Cauley JA, Ensrud KE, Lewis CE, Barrett-Connor E, Schwartz AV, Lee DC, Bhasin S. Effect of Testosterone treatment on volumetric bone density and strength in older men with low Testosterone: a controlled clinical trial. JAMA internal medicine. 2017 Apr 1;177(4):471-9.
  12. Kaminetsky, J.C., et al., A 52-week study of dose adjusted subcutaneous Testosterone enanthate in oil self-administered via disposable auto-injector. The Journal of Urology, 2019. 201(3): p. 587-594.
  13. Barqawi A, Crawford ED. Testosterone replacement therapy and the risk of prostate cancer. Is there a link?. International journal of impotence research. 2006 Jul;18(4):323-8.


Further reading

Understanding the Importance of Regular Testosterone Checks

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